LeuDH
| PRODUCT DESCRIPTION | |
| Catalog No.: | SDZ500280 |
| Appearance: | White amorphous powder |
| Source: | Microorganism |
| Enzyme Commission Number: | EC 1.4.1.9 |
| CAS Number: | 9082-71-7 |
| Storage temperature: | -20℃ |
| Specific activity: | ≥ 25U/mg protein |
| Unit definition: | One unit will convert one micromole of L-Leucine to αKetoisocaproate per minute at pH 10.5 at 37℃ |
| PROPERTIES | |
| Molecular weight: | 43 kDa (SDS-PAGE) |
| Isoelectric point: | 6.6 |
| Michaelis constant: | 2.6×10-4 M (NAD ) |
| 2.0×10-3 M(L-Leucine) | |
| 6.8×10-4 M(α-Ketoisocaproate) | |
| 4.2×10-2 M (NH3) | |
| 2.3×10-4 M (NADH) | |
| Optimum pH: | 11.0(L-Leu→α-K I C) |
| 8.5(α-K I C→ L-Leu ) | |
| Optimum temperature: | 50-60℃(L-Leu →α-K I C) |
| 60~70℃ (α-K I C→ L-Leu ) | |
| pH Stability: | 6.0~11.0 (25℃, 15hr) |
| Thermal stability: | < 55℃ (pH 7.0, 20min) |
| Inhibitors: | Fe3+ ,NEM,Proclin,SDS |
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中文名称：亮氨酸脱氢酶
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英文名称：Leucine Dehydrogenase
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CAS：9082-71-7
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酶活：≥ 25U/mg protein
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活性：One unit will convert one micromole of L-Leucine to αKetoisocaproate per minute at pH 10.5 at 37℃
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